Using Bioluminescent Technologies To Monitor Intracellular Physiology

Using Bioluminescent Technologies To Monitor Intracellular Physiology: Applications to Apoptosis, Cell Viability, Cell Signaling, and Gene Expression Analysis  

In order to understand complex biological systems it is often necessary to quantify changes in the key intracellular physiological parameters that control cellular behavior.  The complexity of cells requires technologies that can delineate between the thousands of biochemical entities residing inside of cells.  Photonic emission from bioluminescent reactions provides a uniquely effective tool for accurately quantifying biologically relevant changes in cell based assays.  The original applications of bioluminescence were primarily related to understanding gene expression changes due to promoter structure variation.  Subsequent evolution of the technology has expanded its use to a wide variety applications for doing experiments in tissue culture including assaying cell viability, apoptosis and cell signaling.  Recent developments in multiplexing technologies have helped researchers better understand the mechanisms of cell death and the control of apoptotic activation.

Visitors :

John Watson, Marketing director, Promega Cooperation, from Madison , WI . USA .

Frank Fan, Senior scientist, Promega Cooperation, from Madison , WI . USA .

Helen Fu, Sales and marketing manager, Promega Beijing Branch, from Beijing .

Judy Dong , Field application specialist, Promega Beijing Branch, from Guangzhou .