2002.09-2006.07 青岛农业大学 动物医学学士

2006.09-2009.07  华南农业大学 临床兽医学硕士    

2009/07—至今 中国科学院广州生物医药与健康研究院助理研究员，高级实验师

基因编辑动物模型的建立及其行为学和影像学分析与干细胞及基因治疗研究；

褪黑素和氨基胍对内毒素血症山羊心肌及其线粒体细胞凋亡水平的影响

以CRISPR/Cas9 系统打靶PINK/PARK双基因制备人类帕金森疾病猪模型，2016A030313169，广东省自然科学基金，主持

老年痴呆猪模型发病早期中期晚期行为学及核磁影像学评价标准的建立与干细胞治疗，2019A030317010，广东省自然科学基金，主持

参与获得广州市科技进步1等奖；

参与获得广东省科技进步1等奖；

中国科学院广州生物医药与健康研究院先进工作者；

国家首批执业兽医师；

南粤优秀研究生；

山东省省级三好学生；

山东省省级优秀毕业生；

1.  Yan S^#, Tu Z^#, Liu Z^#（共同一作）, Fan N, Yang H, Yang S, Yang W, Zhao Y, Ouyang Z, Lai C, Yang H, Li L, Liu Q, Shi H, Xu G, Zhao H, Wei H, Pei Z, Li S^＊, Lai L^＊, Li XJ^＊.A Huntingtin Knockin Pig Model Recapitulates Features of Selective Neurodegeneration in Huntington's Disease.Cell. 2018 May 3;173(4):989-1002.

2．Yang D^#, Yang H^#, Li W, Zhao B, Ouyang Z, Liu Z, Zhao Y, Fan N, Song J, Tian J, Li F, Zhang J, Chang L, Pei D, Chen YE^＊, Lai L^＊. Generation of PPARγ mono-allelic knockout pigs via zinc-finger nucleases and nuclear transfer cloning. Cell Res. 2011Jun;21(6):979-82.

3．Yang H^#, Wang G^#, Sun H^#, Shu R^#, Liu T, Wang CE, Liu Z, Zhao Y, Zhao B, Ouyang Z, Yang D, Huang J, Zhou Y, Li S, Jiang X^＊, Xiao Z^＊, Li XJ^＊, Lai L^＊. Species-dependent neuropathology in transgenic SOD1 pigs. Cell Res. 2014 Apr;24(4):464-81.

4．Li X^#, Yang Y^#, Bu L, Guo X, Tang C, Song J, Fan N, Zhao B, Ouyang Z, Liu Z, Zhao Y, Yi X, Quan L, Liu S, Yang Z, Ouyang H, Chen YE, Wang Z^＊, Lai L^＊. Rosa26-targeted swine models for stable gene over-expression and Cre-mediated lineage tracing. Cell Res. 2014 Apr;24(4):501-4. Epub 2014 Feb 7.

5．Fan N^#, Chen J^#, Shang Z^#, Dou H, Ji G, Zou Q, Wu L, He L, Wang F, Liu K, Liu N, Han J, Zhou Q, Pan D, Yang D, Zhao B, Ouyang Z, Liu Z, Zhao Y, Lin L, Zhong C, Wang Q, Wang S, Xu Y, Luan J, Liang Y, Yang Z, Li J, Lu C, Vajta G, Li Z, Ouyang H, Wang H, Wang Y, Yang Y, Liu Z, Wei H, Luan Z, Esteban MA, Deng H, Yang H, Pei D, Li N, Pei G, Liu L, Du Y^＊, Xiao L^＊, Lai L^＊. Piglets cloned from induced pluripotent stem cells. Cell Res. 2013 Jan;23 (1):162-6.

6. Xie J^#, Ge W^#, Li N^#, Liu Q^#, Chen F, Yang X, Huang X, Ouyang Z, Zhang Q, Zhao Y, Liu Z, Gou S, Wu H, Lai C, Fan N, Jin Q, Shi H, Liang Y, Lan T, Quan L, Li X, Wang K^＊, Lai L^＊. Efficient base editing for multiple genes and loci in pigs using base editors. Nat Commun. 2019 Jun 28;10(1):2852.

7．Wang G^#, Yang H^#, Yan S^#, Wang CE, Liu X, Zhao B, Ouyang Z, Yin P, Liu Z, Zhao Y, Liu T, Fan N, Guo L, Li S^＊, Li XJ^＊, Lai L^＊. Cytoplasmic mislocalization of RNA splicing factors and aberrant neuronal gene splicing in TDP-43 transgenic pig brain. Mol Neurodegener. 2015 Sep 3;10:42.

8. Wang K^#, Jin Q^#, Ruan D^#, Yang Y, Liu Q, Wu H, Zhou Z, Ouyang Z, Liu Z, Zhao Y, Zhao B, Zhang Q, Peng J, Lai C, Fan N, Liang Y, Lan T, Li N, Wang X, Wang X, Fan Y, Doevendans PA, Sluijter JPG, Liu P^＊, Li X^＊, Lai L^＊. Cre-dependent Cas9-expressing pigs enable efficient in vivo genome editing.Genome Res. 2017 Dec;27(12):2061-2071.

9.  Wu H^#, Liu Q^#, Shi H, Xie J, Zhang Q, Ouyang Z, Li N, Yang Y, Liu Z, Zhao Y, Lai C, Ruan D, Peng J, Ge W, Chen F, Fan N, Jin Q, Liang Y, Lan T, Yang X, Wang X, Lei Z, Doevendans PA, Sluijter JPG, Wang K^＊, Li X^＊, Lai L^＊. Engineering CRISPR/Cpf1 with tRNA promotes genome editing capability in mammalian systems.Cell Mol Life Sci. 2018 Oct;75(19):3593-3607.

10．Zhou X^#, Xin J^#, Fan N, Zou Q, Huang J, Ouyang Z, Zhao Y, Zhao B, Liu Z, Lai S, Yi X, Guo L, Esteban MA, Zeng Y, Yang H^＊, Lai L^＊. Generation of CRISPR/Cas9-mediated gene-targeted pigs via somatic cell nuclear transfer. Cell Mol Life Sci. 2015 Mar;72(6):1175-84. Epub 2014 Oct 2.

11.  Ruan D^#, Peng J^#, Wang X^#, Ouyang Z^#, Zou Q, Yang Y, Chen F, Ge W, Wu H, Liu Z, Zhao Y, Zhao B, Zhang Q, Lai C, Fan N, Zhou Z, Liu Q, Li N, Jin Q, Shi H, Xie J, Song H, Yang X, Chen J, Wang K^＊, Li X^＊, Lai L^＊. XIST Derepression in Active X Chromosome Hinders Pig Somatic Cell Nuclear Transfer.Stem Cell Reports. 2018 Feb 13;10(2):494-508.

12.  Jin Q, Yang X, Gou S, Liu X, Zhuang Z, Liang Y, Shi H, Huang J, Wu H, Zhao Y, Ouyang Z, Zhang Q, Liu Z, Chen F, Ge W, Xie J, Li N, Lai C, Zhao X, Wang J, Lian M, Li L, Quan L, Ye Y, Lai L, Wang K. Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression. Sci China Life Sci. 2022 May 18.

13.  Yang Y^#, Wang K^#, Wu H, Jin Q, Ruan D, Ouyang Z, Zhao B, Liu Z, Zhao Y, Zhang Q, Fan N, Liu Q, Guo S, Bu L, Fan Y, Sun X, Li X^＊, Lai L^＊.Genetically humanized pigs exclusively expressing human insulin are generated through custom endonuclease-mediated seamless engineering.J Mol Cell Biol. 2016 Apr;8(2):174-7.

14．Zou Q^#, Wang X^#, Liu Y^#, Ouyang Z^#, Long H, Wei S, Xin J, Zhao B, Lai S, Shen J, Ni Q, Yang H, Zhong H, Li L, Hu M, Zhang Q, Zhou Z, He J, Yan Q, Fan N, Zhao Y, Liu Z, Guo L, Huang J, Zhang G, Ying J^＊, Lai L^＊, Gao X^＊. Generation of gene-target dogs using CRISPR/Cas9 system. J Mol Cell Biol. 2015 Dec;7(6):580-3. doi: 10.1093

15． Yang D^#, Wang CE^#, Zhao B, Li W, Ouyang Z, Liu Z, Yang H, Fan P, O'Neill A, Gu W, Yi H, Li S, Lai L^＊,Li XJ^＊. Expression of Huntington's disease protein results in apoptotic neurons in the brains of cloned transgenic pigs. Hum Mol Genet.19 (20): 3983-94.

16.  Han K^#, Liang L^#, Li L^#, Ouyang Z, Zhao B, Wang Q, Liu Z, Zhao Y, Ren X, Jiang F, Lai C, Wang K, Yan S, Huang L, Guo L, Zeng K^＊, Lai L^＊, Fan N^＊.Generation of Hoxc13 knockout pigs recapitulates human ectodermal dysplasia-9.Hum Mol Genet. 2017 Jan 1;26(1):184-191.

17.  Huang J^#, Guo X^#, Fan N, Song J, Zhao B, Ouyang Z, Liu Z, Zhao Y, Yan Q, Yi X, Schambach A, Frampton J, Esteban MA, Yang D, Yang H^＊, Lai L＊. RAG1/2 knockout pigs with severe combined immunodeficiency. J Immunol. 2014 Aug 1;193(3): 1496-503.

18.  Li W^#, Shi L^#, Zhuang Z^#, Wu H^#, Lian M, Chen Y, Li L, Ge W, Jin Q, Zhang Q, Zhao Y, Liu Z, Ouyang Z, Ye Y, Li Y, Wang H, Liao Y, Quan L, Xiao L, Lai L^＊, Meng G^＊, Wang K^＊.  Engineered Pigs Carrying a Gain-of-Function NLRP3 Homozygous Mutation Can Survive to Adulthood and Accurately Recapitulate Human Systemic Spontaneous Inflammatory Responses. J Immunol. 2020 Nov 1;205(9):2532-2544. doi:10.4049/jimmunol.1901468. Epub 2020 Sep 21.PMID: 32958688

19.  Lan T^#, Zheng Y^#, Suo Y, Wei Y, Shi H, Yan Q, Zhuang Z, Chen H, Zhang Q, Fan N, Zhao Y, Ouyang Z, Lai C, Liu Z, Zhou J, Tang C, Kim NH, Zou Q^＊, Wang X^＊. Generation of pigs with humanized type II collagen by precise human COL2A1 gene knock-in. J Genet Genomics. 2022 May 19:S1673-8527(22)00139-4.

20.  Chen P^#, Gao H^#, Lu Y, Nie H, Liu Z, Zhao Y, Fan N, Zou Q, Dai Y, Tang A, Hara H, Cai Z, Cooper DKC, Lai L＊, Mou L^＊.Altered expression of eNOS, prostacyclin synthase, prostaglandin G/H synthase, and thromboxane synthase in porcine aortic endothelial cells after exposure to human serum-relevance to xenotransplantation.Cell Biol Int. 2017 Jul;41(7):798-808.

21． Lai S^#, Wei S, Zhao B, Ouyang Z, Zhang Q, Fan N, Liu Z, Zhao Y, Yan Q, Zhou X, Li L, Xin J, Zeng Y^＊, Lai L^＊, Zou Q.Generation of Knock-In Pigs Carrying Oct4-tdTomato Reporter through CRISPR/Cas9-Mediated Genome Engineering. PLoS One. 2016 Jan 12;11(1):e0146562.

22． Deng W^#, Yang D^#, Zhao B, Ouyang Z, Song J, Fan N, Liu Z, Zhao Y, Wu Q, Nashun B, Tang J, Wu Z, Gu W^＊, Lai L^＊. Use of the 2A peptide for generation of multi-transgenic pigs through a single round of nuclear transfer. PLoS One. 2011;6(5):e19986. Epub 2011 May 13.

23.  Xin J^#, Yang H^#, Fan N, Zhao B, Ouyang Z, Liu Z, Zhao Y, Li X, Song J, Yang Y, Zou Q, Yan Q, Zeng Y^＊, Lai L^＊.Highly Efficient Generation of GGTA1 Biallelic Knockout Inbred Mini-Pigs with TALENs. PLoS One. 2013 Dec 178(12)

24.  Yong Wang^#, Hua-Qiang Yang^#, Wen Jiang, Na-Na Fan, Ben-Tian Zhao, Zhen Ou-Yang, Zhao-Ming Liu, Yu Zhao, Dong-Shan Yang, Xiao-Yang Zhou, Hai-Tao Shang, Lu-Lu Wang, Peng-Ying Xiang, Liang-Peng Ge, Hong Wei^＊, Liang-Xue Lai^＊. Transgenic expression of human cytoxic T-lymphocyte associated antigen4-immunoglobulin (hCTLA4Ig) by porcine skin for xenogeneic skin grafting. Transgenic Res. 2015 Apr;24(2):199-211.

25. Yan Q^#, Yang H, Yang D, Zhao B, Ouyang Z, Liu Z, Fan N, Ouyang H, Gu W, Lai L^＊. Production of transgenic pigs over-expressing the antiviral gene Mx1. Cell Regen . 2014 Sep 26;3(1)

26．Liu Zhao-ming，Wu Caixia，Yang Hong, Li Ying,Pan Jia-qiang,Li Peng-fei,Guo Ming-sheng,Liu nian,Zeng Jian-ying,Tang Zhao-xin^＊. Protective Effects of Melatonin on Myocardial No Metabolism and Sulfhydryl in Goats with Endotoxemia. 中国首届兽医临床大会暨中国畜牧兽医学年会优秀论文奖.

27．LIU Zhao-ming,YANG Hong,SHI Da-you,LIU Jing,GUO Jian-ying,LI Ying,PAN Jia-qiang,TANG Zhao-xin^＊. Protective effects of aminoguanidin on Myocardial mitochondrial lipid peroxidation damage and the activity of ATPase in goats with endotoxemia. 中国第二届兽医临床大会暨中国畜牧兽医学内科年会优秀论文奖.