| 论文编号: |
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| 第一作者所在部门: |
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| 中文论文题目: |
AGBE: a dual deaminase-mediated base editor by fusing CGBE with ABE for creating a saturated mutant population with multiple editing patterns |
| 英文论文题目: |
AGBE: a dual deaminase-mediated base editor by fusing CGBE with ABE for creating a saturated mutant population with multiple editing patterns |
| 作者: |
Yanhui Liang,Jingke Xie,Quanjun Zhang, Xiaomin Wang1,Shixue Gou1,Lihui Lin1,Tao Chen,Weikai Ge1,Zhenpeng Zhuang,Meng Lian,Fangbing Chen1, Nan Li,ZhenOuyang,Chengdan Lai,Xiaoyi Liu1,Lei Li1,Yinghua Ye,HanWu,Kepin Wang,Liangxue Lai |
| 论文出处: |
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| 刊物名称: |
Nucleic Acids Research |
| 年: |
2022 |
| 卷: |
50 |
| 期: |
9 |
| 页: |
5384-5399 |
| 联系作者: |
Liangxue Lai |
| 收录类别: |
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| 影响因子: |
19.160 |
| 摘要: |
Establishing saturated mutagenesis in a specific gene through gene editing is an efficient approach for identifying the relationships between mutations and the corresponding phenotypes. CRISPR/Cas9-based sgRNA library screening often creates indel mutations with multiple nucleotides. Single base editors and dual deaminase-mediated base editors can achieve only one and two types of base substitutions, respectively. A new glycosylase base editor (CGBE) system, in which the uracil glycosylase inhibitor (UGI) is replaced with uracil-DNA glycosylase (UNG), was recently reported to efficiently induce multiple base conversions, including C-to-G, C-to-T and C-to-A. In this study, we fused a CGBE with ABE to develop a new type of dual deaminase-mediated base editing system, the AGBE system, that can simultaneously introduce 4 types of base conversions (C-to-G, C-to-T, C-to-A and A-to-G) as well as indels with a single sgRNA in mammalian cells. AGBEs can be used to establish saturated mutant populations for verification of the functions and consequences of multiple gene mutation patterns, including single-nucleotide variants (SNVs) and indels, through high-throughput screening. |
| 英文摘要: |
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